Analyse multi-échelle des interactions physico-chimiques et biologiques entre des nanoparticules manufacturées et des bactéries

Project Information

Principal Investigator	Antoine Thill
Institution
Project URL	View
Relevance to Implications	High
Class of Nanomaterial	Engineered Nanomaterials
Impact Sector	Environment
Broad Research Categories	Hazard Response Generation, Dispersion, Transformation etc. Characterization Risk Assessment
NNI identifier

Funding Information

Country	France
Anticipated Total Funding	$657,805.00
Annual Funding	$328,902.50
Funding Source	ANR, CEA, CNRS
Funding Mechanism	Extramural
Funding Sector	Government
Start Year	2005
Anticipated End Year	2007

Abstract/Summary

“Physico-chemical and biological interaction between three bacteria (Ralstonia, Syncechocystis, E.coli) and five nanoparticles (Fe2O3, TiO2, CeO2, ZnO et fullerènes) will be studied. The dispersion state of the nanoparticles before contact will be controlled (surface charge modification and/or organic molecules adsorption). The chemical interaction will be followed at the atomic scale through XAS and Mossbauer experiments . The localization of the nanoparticles after exposure will be measured at the local scale with TEM, Small Angle X-ray Scattering, light scattering and confocal microsopy. From the biological point of view, the cytotoxicity, the genotoxicity and the nanoparticles influence on the three bacteria metabolism will be assessed by survival tests, proteomic analysis and genomic analysis. The central goal of the project is to establish an in situ relationship between the properties of the nanoparticles and their biological impact.”